Hotspot

ycf4-cemA

LSC · 61084-62014 · 931 bp

Back to Trithuria

Pi 0.106

MarkerSeek score 82.20

Primer available yes

Label ycf4-cemA

Feature Metadata

label_name: ycf4-cemA
feature_type: igs
region: LSC
length_bp: 931
variable_sites: 96
pi: 0.106
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.97
markerseek_score: 82.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf4-cemA_p1	TTTCGTTGGGGATTTCCTGG	ACCGGAAAAGCAGAAACGAT	50.0	45.0	58.1	58.1	1755-1770 bp	1.0	85.4
ycf4-cemA_p2	TATGGAAATAGGAGGCCGGT	ACCGGAAAAGCAGAAACGAT	50.0	45.0	57.9	58.1	1639-1654 bp	1.0	85.4
ycf4-cemA_p3	TGGAAATAGGAGGCCGGTTA	ACCGGAAAAGCAGAAACGAT	50.0	45.0	58.1	58.1	1637-1652 bp	1.0	85.3
ycf4-cemA_p4	TCCCCGTCGTGTCCTTTATA	ACCGGAAAAGCAGAAACGAT	50.0	45.0	57.9	58.1	1657-1672 bp	1.0	85.1
ycf4-cemA_p5	TGGCGATCAGAACACGTATG	ACCGGAAAAGCAGAAACGAT	50.0	45.0	58.1	58.1	2061-2076 bp	1.0	84.9