Hotspot

ycf3-tRNA-Ser(GGA)

LSC · 44765-45482 · 718 bp

Back to Trithuria

Pi 0.0876

MarkerSeek score 80.90

Primer available yes

Label ycf3-tRNA-Ser(GGA)

Feature Metadata

label_name: ycf3-tRNA-Ser(GGA)
feature_type: igs
region: LSC
length_bp: 718
variable_sites: 61
pi: 0.0876
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.97
markerseek_score: 80.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf3-tRNA-Ser(GGA)_p1	TTGGTAGTCCCCATCCCAAT	ACGGCCGTGTAGTGGATATA	50.0	50.0	58.0	58.0	1818-1887 bp	1.0	86.4
ycf3-tRNA-Ser(GGA)_p2	TTGGTAGTCCCCATCCCAAT	GTTGCGTATGGCTTCCACTA	50.0	50.0	58.0	58.0	1883-1952 bp	1.0	86.1
ycf3-tRNA-Ser(GGA)_p3	TTGGTAGTCCCCATCCCAAT	TGGCTTCCACTATTCCTGGA	50.0	50.0	58.0	58.0	1875-1944 bp	1.0	86.0
ycf3-tRNA-Ser(GGA)_p4	TTGGTAGTCCCCATCCCAAT	GCTTCCACTATTCCTGGAGC	50.0	55.0	58.0	58.0	1873-1942 bp	1.0	86.0
ycf3-tRNA-Ser(GGA)_p5	TTGGTAGTCCCCATCCCAAT	GGAGCCCGCCAATTAGTTAA	50.0	50.0	58.0	58.0	1858-1927 bp	1.0	86.0