Hotspot

tRNA-Ser(UGA)-psbZ

LSC · 36198-36536 · 339 bp

Back to Trithuria

Pi 0.0683

MarkerSeek score 80.70

Primer available yes

Label tRNA-Ser(UGA)-psbZ

Feature Metadata

label_name: tRNA-Ser(UGA)-psbZ
feature_type: igs
region: LSC
length_bp: 339
variable_sites: 22
pi: 0.0683
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.95
markerseek_score: 80.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
tRNA-Ser(UGA)-psbZ_p1	CAACCACTCAGCCATCTCTC	TGCAAAAACAGCCAATTGGA	55.0	40.0	58.0	57.0	376-393 bp	1.0	80.0
tRNA-Ser(UGA)-psbZ_p2	ACGAGCAAAGCAAAAGGAGA	TGCAAAAACAGCCAATTGGA	45.0	40.0	58.0	57.0	457-474 bp	1.0	78.5
tRNA-Ser(UGA)-psbZ_p3	CAACCACTCAGCCATCTCTC	TGCAAAAACAGCCAATTGGAA	55.0	38.1	58.0	57.7	376-393 bp	1.0	77.8
tRNA-Ser(UGA)-psbZ_p4	CAACCACTCAGCCATCTCTC	AACAGCCAATTGGAAAGCAA	55.0	40.0	58.0	56.7	370-387 bp	1.0	77.7
tRNA-Ser(UGA)-psbZ_p5	ACGAGCAAAGCAAAAGGAGA	TGCAAAAACAGCCAATTGGAA	45.0	38.1	58.0	57.7	457-474 bp	1.0	76.3