Hotspot

petN-psbM

LSC · 28658-29950 · 1293 bp

Back to Trithuria

Pi 0.0852

MarkerSeek score 82.60

Primer available yes

Label petN-psbM

Feature Metadata

label_name: petN-psbM
feature_type: igs
region: LSC
length_bp: 1293
variable_sites: 87
pi: 0.0852
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.79
markerseek_score: 82.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
petN-psbM_p1	AATAAGTCTCGCTTGGGCTG	ATTGCTACTGCGCTGTTCAT	50.0	45.0	58.0	58.3	1207-1456 bp	1.0	86.9
petN-psbM_p2	GGGGTAGAAGTGGGCTCTAA	ATTGCTACTGCGCTGTTCAT	55.0	45.0	58.1	58.3	1145-1394 bp	1.0	86.4
petN-psbM_p3	AATAAGTCTCGCTTGGGCTG	TATTGCTACTGCGCTGTTCA	50.0	45.0	58.0	57.6	1208-1457 bp	1.0	86.0
petN-psbM_p4	TATGGGGTAGAAGTGGGCTC	ATTGCTACTGCGCTGTTCAT	55.0	45.0	58.2	58.3	1148-1397 bp	1.0	85.8
petN-psbM_p5	GGGGTAGAAGTGGGCTCTAA	TATTGCTACTGCGCTGTTCA	55.0	45.0	58.1	57.6	1146-1395 bp	1.0	85.5