Hotspot

tRNA-Ser(GCU)-tRNA-Gly(GCC)

LSC · 8365-9052 · 688 bp

Back to Trithuria

Pi 0.0752

MarkerSeek score 80.00

Primer available yes

Label tRNA-Ser(GCU)-tRNA-Gly(GCC)

Feature Metadata

label_name: tRNA-Ser(GCU)-tRNA-Gly(GCC)
feature_type: igs
region: LSC
length_bp: 688
variable_sites: 51
pi: 0.0752
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 80.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
tRNA-Ser(GCU)-tRNA-Gly(GCC)_p1	GCTTTAGTCCACTCAGCCAT	ACGAATCACACTTTTACCACT	50.0	38.1	57.6	55.5	748-754 bp	1.0	54.9
tRNA-Ser(GCU)-tRNA-Gly(GCC)_p2	AGTCCACTCAGCCATCTCTC	ACGAATCACACTTTTACCACT	55.0	38.1	58.5	55.5	743-749 bp	1.0	54.1
tRNA-Ser(GCU)-tRNA-Gly(GCC)_p3	GCTTTAGTCCACTCAGCCAT	GAACGAATCACACTTTTACCACT	50.0	39.1	57.6	57.3	750-756 bp	1.0	53.9
tRNA-Ser(GCU)-tRNA-Gly(GCC)_p4	GCTTTAGTCCACTCAGCCAT	AGAACGAATCACACTTTTACCAC	50.0	39.1	57.6	57.3	751-757 bp	1.0	53.8
tRNA-Ser(GCU)-tRNA-Gly(GCC)_p5	AGTCCACTCAGCCATCTCTC	GAACGAATCACACTTTTACCACT	55.0	39.1	58.5	57.3	745-751 bp	1.0	53.0