Hotspot

ycf1

SSC · 126899-132574 · 5676 bp

Back to Trithrinax

Pi 0.00161

MarkerSeek score 54.60

Primer available yes

Label ycf1

Feature Metadata

label_name: ycf1
feature_type: gene
region: SSC
length_bp: 5676
variable_sites: 9
pi: 0.00161
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 54.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf1_p1	CTGCTTCCTTCTTTTTCTTGGA	ACAATTACGCGGGATCAGAC	40.9	50.0	56.8	58.1	128-200 bp	1.0	59.4
ycf1_p2	TCTGCTTCCTTCTTTTTCTTGG	ACAATTACGCGGGATCAGAC	40.9	50.0	56.8	58.1	129-201 bp	1.0	59.4
ycf1_p3	CTGCTTCCTTCTTTTTCTTGGA	CAATTACGCGGGATCAGACA	40.9	50.0	56.8	58.1	127-199 bp	1.0	59.4
ycf1_p4	TCTGCTTCCTTCTTTTTCTTGG	CAATTACGCGGGATCAGACA	40.9	50.0	56.8	58.1	128-200 bp	1.0	59.4
ycf1_p5	TCTGCTTCCTTCTTTTTCTTGGA	ACAATTACGCGGGATCAGAC	39.1	50.0	58.2	58.1	129-201 bp	1.0	59.3