Hotspot

atpB

LSC · 55292-56788 · 1497 bp

Back to Trithrinax

Pi 0.00134

MarkerSeek score 59.50

Primer available yes

Label atpB

Feature Metadata

label_name: atpB
feature_type: gene
region: LSC
length_bp: 1497
variable_sites: 2
pi: 0.00134
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 59.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
atpB_p1	CGTGGTTTGGTAATACGCCA	CGATCAACTTGCTATCGGACA	50.0	47.6	58.6	58.2	1692 bp	1.0	66.1
atpB_p2	GGGGCGTGGTTTGGTAATAC	CGATCAACTTGCTATCGGACA	55.0	47.6	58.9	58.2	1696 bp	1.0	63.3
atpB_p3	CGTGGTTTGGTAATACGCCA	CGATCAACTTGCTATCGGAC	50.0	50.0	58.6	56.4	1692 bp	1.0	63.1
atpB_p4	AGGGGCGTGGTTTGGTAATA	CGATCAACTTGCTATCGGACA	50.0	47.6	59.0	58.2	1697 bp	1.0	62.5
atpB_p5	TAGGGGCGTGGTTTGGTAAT	CGATCAACTTGCTATCGGACA	50.0	47.6	59.0	58.2	1698 bp	1.0	62.5