Hotspot

rpl32-trnL-UAG

SSC · 116416-117187 · 772 bp

Back to Trithrinax

Pi 0.0026
MarkerSeek score 60.20
Primer available yes
Label rpl32-trnL-UAG

Feature Metadata

label_name
rpl32-trnL-UAG
feature_type
igs
region
SSC
length_bp
772
variable_sites
2
pi
0.0026
unique_haplotype_count
2
primer_available
yes
alignment_reliability
1.00
markerseek_score
60.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rpl32-trnL-UAG_p1 GCTCTTTCTTTAGCGAAATCGG AGCGTGTCTACCGATTTCAC 45.5 50.0 58.1 58.0 885 bp 1.0 63.6
rpl32-trnL-UAG_p2 GCTCTTTCTTTAGCGAAATCGG CAGCGTGTCTACCGATTTCA 45.5 50.0 58.1 58.0 886 bp 1.0 63.5
rpl32-trnL-UAG_p3 GCTCTTTCTTTAGCGAAATCGG TCCTAAGAGCAGCGTGTCTA 45.5 50.0 58.1 57.9 895 bp 1.0 62.6
rpl32-trnL-UAG_p4 GCTCTTTCTTTAGCGAAATCGG CCTAAGAGCAGCGTGTCTAC 45.5 55.0 58.1 57.8 894 bp 1.0 62.0
rpl32-trnL-UAG_p5 GCTCTTTCTTTAGCGAAATCGG CTAAGAGCAGCGTGTCTACC 45.5 55.0 58.1 57.8 893 bp 1.0 62.0