Hotspot

atpH-atpI

LSC · 14475-15409 · 935 bp

Back to Trithrinax

Pi 0.00223

MarkerSeek score 52.20

Primer available yes

Label atpH-atpI

Feature Metadata

label_name: atpH-atpI
feature_type: igs
region: LSC
length_bp: 935
variable_sites: 2
pi: 0.00223
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.96
markerseek_score: 52.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
atpH-atpI_p1	ATAACGGAAGCGGCAGAAAT	TAGGTGAATCCATGGAGGGT	45.0	50.0	58.0	57.4	960-996 bp	1.0	78.4
atpH-atpI_p2	ATAACGGAAGCGGCAGAAAT	ATTTTTGCAACGTTAGCCGC	45.0	45.0	58.0	58.6	988-1024 bp	1.0	78.3
atpH-atpI_p3	AATAACGGAAGCGGCAGAAA	TAGGTGAATCCATGGAGGGT	45.0	50.0	58.2	57.4	961-997 bp	1.0	77.3
atpH-atpI_p4	AATAACGGAAGCGGCAGAAA	ATTTTTGCAACGTTAGCCGC	45.0	45.0	58.2	58.6	989-1025 bp	1.0	77.2
atpH-atpI_p5	ATAACGGAAGCGGCAGAAAT	CCGCGGCTTATATAGGTGAA	45.0	50.0	58.0	57.3	972-1008 bp	1.0	77.2