Hotspot

rpl2-trnH-GUG

IRa · 138526-138580 · 55 bp

Back to Tristachya

Pi 0.0182

MarkerSeek score 63.00

Primer available yes

Label rpl2-trnH-GUG

Feature Metadata

label_name: rpl2-trnH-GUG
feature_type: igs
region: IRa
length_bp: 55
variable_sites: 1
pi: 0.0182
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 63.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl2-trnH-GUG_p1	GTTATCCTGCGCTTGGAAGA	CTTTAGCTGGATAAGGGGCG	50.0	55.0	58.0	58.1	223 bp	1.0	79.2
rpl2-trnH-GUG_p2	GTTATCCTGCGCTTGGAAGA	CGGATGTAGCCAAGTGGATC	50.0	55.0	58.0	58.1	205 bp	1.0	79.2
rpl2-trnH-GUG_p3	GTTATCCTGCGCTTGGAAGA	TGGATCAAGGCAGTGGATTG	50.0	50.0	58.0	57.9	191 bp	1.0	79.2
rpl2-trnH-GUG_p4	GTTATCCTGCGCTTGGAAGA	GGATCAAGGCAGTGGATTGT	50.0	50.0	58.0	57.9	190 bp	1.0	79.1
rpl2-trnH-GUG_p5	GTTATCCTGCGCTTGGAAGA	GTGGATCAAGGCAGTGGATT	50.0	50.0	58.0	57.9	192 bp	1.0	79.1