Hotspot

ccsA-ndhD

SSC · 108619-108806 · 188 bp

Back to Tristachya

Pi 0.0372

MarkerSeek score 64.30

Primer available yes

Label ccsA-ndhD

Feature Metadata

label_name: ccsA-ndhD
feature_type: igs
region: SSC
length_bp: 188
variable_sites: 7
pi: 0.0372
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 64.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ccsA-ndhD_p1	TGGTTCATTTACATTACCATCT	TTGACAGGGTAGAGGCTCTC	31.8	55.0	53.1	58.1	260 bp	1.0	42.2
ccsA-ndhD_p2	TGGTTCATTTACATTACCATCT	GACAGGGTAGAGGCTCTCTT	31.8	55.0	53.1	57.8	258 bp	1.0	42.2
ccsA-ndhD_p3	TGGTTCATTTACATTACCATCT	CTCTCGCTATCCGTTGACAG	31.8	55.0	53.1	57.9	273 bp	1.0	42.1
ccsA-ndhD_p4	TGGTTCATTTACATTACCATCT	TCTCTCGCTATCCGTTGACA	31.8	50.0	53.1	58.3	274 bp	1.0	42.0
ccsA-ndhD_p5	TGGTTCATTTACATTACCATCT	CCGGATTTTGTTCTCTCGCT	31.8	50.0	53.1	58.3	285 bp	1.0	42.0