Hotspot

ndhF-rpl32

SSC · 105941-106784 · 844 bp

Back to Tristachya

Pi 0.0368

MarkerSeek score 72.40

Primer available yes

Label ndhF-rpl32

Feature Metadata

label_name: ndhF-rpl32
feature_type: igs
region: SSC
length_bp: 844
variable_sites: 31
pi: 0.0368
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 72.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhF-rpl32_p1	GGAAGTGGGAGAAGAGGGAT	TCCAAAGATTTTTACGAATACGCT	55.0	33.3	58.1	57.3	954-955 bp	1.0	44.0
ndhF-rpl32_p2	GGAAGTGGGAGAAGAGGGAT	TCCAAAGATTTTTACGAATACGC	55.0	34.8	58.1	55.9	954-955 bp	1.0	42.8
ndhF-rpl32_p3	GGAAGTGGGAGAAGAGGGAT	CCAAAGATTTTTACGAATACGCT	55.0	34.8	58.1	55.9	953-954 bp	1.0	42.8
ndhF-rpl32_p4	GGAAGTGGGAGAAGAGGGAT	TCCAAAGATTTTTACGAATACGCTT	55.0	32.0	58.1	57.9	954-955 bp	1.0	42.8
ndhF-rpl32_p5	GGAAGTGGGAGAAGAGGGAT	TTCCAAAGATTTTTACGAATACGCT	55.0	32.0	58.1	57.9	955-956 bp	1.0	42.8