Hotspot

rpl16-rps3

LSC · 79652-79794 · 143 bp

Back to Tristachya

Pi 0.021
MarkerSeek score 63.50
Primer available yes
Label rpl16-rps3

Feature Metadata

label_name
rpl16-rps3
feature_type
igs
region
LSC
length_bp
143
variable_sites
3
pi
0.021
unique_haplotype_count
2
primer_available
yes
alignment_reliability
1.00
markerseek_score
63.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rpl16-rps3_p1 AACGAATCACACACTAAGCA TACCCCTCCAAACAATTCGC 40.0 50.0 55.3 58.2 271-273 bp 1.0 58.1
rpl16-rps3_p2 ACGAATCACACACTAAGCAT TACCCCTCCAAACAATTCGC 40.0 50.0 55.1 58.2 270-272 bp 1.0 56.3
rpl16-rps3_p3 AACGAATCACACACTAAGCAT TACCCCTCCAAACAATTCGC 38.1 50.0 55.9 58.2 271-273 bp 1.0 54.7
rpl16-rps3_p4 ACTAACGAATCACACACTAAGCA TACCCCTCCAAACAATTCGC 39.1 50.0 58.1 58.2 274-276 bp 1.0 54.5
rpl16-rps3_p5 AACGAATCACACACTAAGCA CTCCAAACAATTCGCGCTAA 40.0 45.0 55.3 57.1 266-268 bp 1.0 52.4