Hotspot

psaA-ycf3

LSC · 41975-42625 · 651 bp

Back to Tristachya

Pi 0.02

MarkerSeek score 56.00

Primer available yes

Label psaA-ycf3

Feature Metadata

label_name: psaA-ycf3
feature_type: igs
region: LSC
length_bp: 651
variable_sites: 13
pi: 0.02
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 56.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psaA-ycf3_p1	CCGCCTACATCACCATTCAA	TAGAGCCGATCCACTAAGGG	50.0	55.0	58.0	58.0	1614-1620 bp	1.0	80.8
psaA-ycf3_p2	GCATCAGCATGTAGGTTCCA	TAGAGCCGATCCACTAAGGG	50.0	55.0	58.0	58.0	1383-1389 bp	1.0	80.8
psaA-ycf3_p3	GTTATTCCAGATGCTCGCCA	TAGAGCCGATCCACTAAGGG	50.0	55.0	58.0	58.0	1677-1683 bp	1.0	80.7
psaA-ycf3_p4	CCAAGTCCTAATAACCCCGC	TAGAGCCGATCCACTAAGGG	55.0	55.0	58.0	58.0	1842-1848 bp	1.0	80.6
psaA-ycf3_p5	CTACATCTTGGAACCAGGCC	TAGAGCCGATCCACTAAGGG	55.0	55.0	58.0	58.0	1799-1805 bp	1.0	80.6