Hotspot

trnD-GUC-psbM

LSC · 15864-16791 · 928 bp

Back to Tristachya

Pi 0.0861
MarkerSeek score 78.90
Primer available yes
Label trnD-GUC-psbM

Feature Metadata

label_name
trnD-GUC-psbM
feature_type
igs
region
LSC
length_bp
928
variable_sites
79
pi
0.0861
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.99
markerseek_score
78.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnD-GUC-psbM_p1 CTTAGAACACCGCAGACGAA TCTCCCCTTTCGTGGATTCT 50.0 50.0 57.9 58.0 1496-1620 bp 1.0 84.4
trnD-GUC-psbM_p2 TTCTTAGAACACCGCAGACG TCTCCCCTTTCGTGGATTCT 50.0 50.0 57.9 58.0 1498-1622 bp 1.0 84.4
trnD-GUC-psbM_p3 AATTCTTCCTGGGTCGATGC TCTCCCCTTTCGTGGATTCT 50.0 50.0 58.0 58.0 1862-1985 bp 1.0 84.0
trnD-GUC-psbM_p4 CGCTATCGTCTAGTGATGCC TCTCCCCTTTCGTGGATTCT 55.0 50.0 58.0 58.0 2003-2126 bp 1.0 83.8
trnD-GUC-psbM_p5 GGCGCTATCGTCTAGTGATG TCTCCCCTTTCGTGGATTCT 55.0 50.0 58.0 58.0 2005-2128 bp 1.0 83.8