Hotspot

psbC-trnS-UGA

LSC · 11519-11666 · 148 bp

Back to Tristachya

Pi 0.0233

MarkerSeek score 61.50

Primer available yes

Label psbC-trnS-UGA

Feature Metadata

label_name: psbC-trnS-UGA
feature_type: igs
region: LSC
length_bp: 148
variable_sites: 3
pi: 0.0233
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.87
markerseek_score: 61.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbC-trnS-UGA_p1	CATTTGTGGCATGCAGGAAG	GAGAGATGGCTGAGTGGTTG	50.0	55.0	58.0	58.0	319-337 bp	1.0	84.5
psbC-trnS-UGA_p2	CATTTGTGGCATGCAGGAAG	GATTGGGCTGTGGAGAGATG	50.0	55.0	58.0	58.0	331-349 bp	1.0	84.4
psbC-trnS-UGA_p3	CATTTGTGGCATGCAGGAAG	GCTGAGTGGTTGATAGCTCC	50.0	55.0	58.0	58.1	311-329 bp	1.0	84.3
psbC-trnS-UGA_p4	CATTTGTGGCATGCAGGAAG	GGCTGAGTGGTTGATAGCTC	50.0	55.0	58.0	58.1	312-330 bp	1.0	84.3
psbC-trnS-UGA_p5	CATTTGTGGCATGCAGGAAG	GGTTGATAGCTCCGGTCTTG	50.0	55.0	58.0	58.1	304-322 bp	1.0	84.3