Hotspot

trnK-UUU-rps16

LSC · 3917-4498 · 582 bp

Back to Tristachya

Pi 0.0207
MarkerSeek score 56.10
Primer available yes
Label trnK-UUU-rps16

Feature Metadata

label_name
trnK-UUU-rps16
feature_type
igs
region
LSC
length_bp
582
variable_sites
12
pi
0.0207
unique_haplotype_count
2
primer_available
yes
alignment_reliability
1.00
markerseek_score
56.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnK-UUU-rps16_p1 CCCCACATCAGGCACTAATC TCGAGCCGTATGAGGAGAAA 55.0 50.0 58.0 58.2 1209-1210 bp 1.0 79.2
trnK-UUU-rps16_p2 CCCCACATCAGGCACTAATC TTCGAGCCGTATGAGGAGAA 55.0 50.0 58.0 58.2 1210-1211 bp 1.0 79.2
trnK-UUU-rps16_p3 CCCCACATCAGGCACTAATC TTTCGAGCCGTATGAGGAGA 55.0 50.0 58.0 58.2 1211-1212 bp 1.0 79.2
trnK-UUU-rps16_p4 TGAGGATCAGTCGTGGTCTT TTCGAGCCGTATGAGGAGAA 50.0 50.0 58.1 58.2 1002-1004 bp 1.0 78.9
trnK-UUU-rps16_p5 TGAGGATCAGTCGTGGTCTT TCGAGCCGTATGAGGAGAAA 50.0 50.0 58.1 58.2 1001-1003 bp 1.0 78.8