Hotspot

ndhF

SSC · 102036-104255 · 2220 bp

Back to Trisetum

Pi 0.0102

MarkerSeek score 49.80

Primer available yes

Label ndhF

Feature Metadata

label_name: ndhF
feature_type: gene
region: SSC
length_bp: 2220
variable_sites: 34
pi: 0.0102
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 49.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhF_p1	AAAATCGCGGAAAACCGAAC	GGTATGGAAGCCTATCCCCT	45.0	55.0	58.0	58.0	2752-2919 bp	1.0	85.0
ndhF_p2	AATCGCGGAAAACCGAACTA	GGTATGGAAGCCTATCCCCT	45.0	55.0	57.9	58.0	2750-2917 bp	1.0	84.7
ndhF_p3	TCGCGGAAAACCGAACTATT	GGTATGGAAGCCTATCCCCT	45.0	55.0	57.9	58.0	2748-2915 bp	1.0	84.7
ndhF_p4	GGGGATAGGAAAGGGGAAGA	GGTATGGAAGCCTATCCCCT	55.0	55.0	57.8	58.0	2690-2864 bp	1.0	83.7
ndhF_p5	GCATGGTGTTCTTCGACTGA	GGTATGGAAGCCTATCCCCT	50.0	55.0	58.2	58.0	2843-2999 bp	0.666667	69.2