Hotspot

ndhG-ndhI

SSC · 110645-110898 · 254 bp

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Pi 0.0241

MarkerSeek score 57.10

Primer available yes

Label ndhG-ndhI

Feature Metadata

label_name: ndhG-ndhI
feature_type: igs
region: SSC
length_bp: 254
variable_sites: 9
pi: 0.0241
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 57.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhG-ndhI_p1	AAGTTGTGCCACAGCTACAA	GAGTACTGTCCGACAAGCTG	45.0	55.0	57.9	58.0	644-653 bp	1.0	82.1
ndhG-ndhI_p2	AGTTGTGCCACAGCTACAAA	GAGTACTGTCCGACAAGCTG	45.0	55.0	57.9	58.0	643-652 bp	1.0	82.1
ndhG-ndhI_p3	TTGTGCCACAGCTACAAAGT	GAGTACTGTCCGACAAGCTG	45.0	55.0	57.9	58.0	641-650 bp	1.0	82.1
ndhG-ndhI_p4	AGAAGTTGTGCCACAGCTAC	GAGTACTGTCCGACAAGCTG	50.0	55.0	58.1	58.0	646-655 bp	1.0	81.5
ndhG-ndhI_p5	TTGTGCCACAGCTACAAAGT	GTGTTCGCGTATGTCCGATA	45.0	50.0	57.9	57.9	766-775 bp	1.0	81.0