Hotspot

ndhF-rpl32

SSC · 104256-105084 · 829 bp

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Pi 0.0238

MarkerSeek score 68.60

Primer available yes

Label ndhF-rpl32

Feature Metadata

label_name: ndhF-rpl32
feature_type: igs
region: SSC
length_bp: 829
variable_sites: 27
pi: 0.0238
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 68.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhF-rpl32_p1	GGAAGTGGGAGAAGAGGGAT	TCCAAATATTTTTACGAATACGCT	55.0	29.2	58.1	55.3	777-979 bp	1.0	46.4
ndhF-rpl32_p2	GGAAGTGGGAGAAGAGGGAT	TCCAAATATTTTTACGAATACGCTT	55.0	28.0	58.1	56.0	777-979 bp	1.0	46.4
ndhF-rpl32_p3	GGAAGTGGGAGAAGAGGGAT	TTCCAAATATTTTTACGAATACGCT	55.0	28.0	58.1	56.0	778-980 bp	1.0	46.4
ndhF-rpl32_p4	GGAAGTGGGAGAAGAGGGAT	TCTTCCAAATATTTTTACGAATACGC	55.0	30.8	58.1	56.9	780-982 bp	1.0	46.4
ndhF-rpl32_p5	AAGGCCAAATCCCATTGACA	TCCAAATATTTTTACGAATACGCT	45.0	29.2	57.7	55.3	805-1007 bp	1.0	46.3