Hotspot

ndhC-trnV-UAC

LSC · 49504-50441 · 938 bp

Back to Trisetum

Pi 0.0168

MarkerSeek score 74.40

Primer available yes

Label ndhC-trnV-UAC

Feature Metadata

label_name: ndhC-trnV-UAC
feature_type: igs
region: LSC
length_bp: 938
variable_sites: 23
pi: 0.0168
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 74.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhC-trnV-UAC_p1	GCCCTGAAATCCAAAATGCC	GGTTCGAATCCGTATAGCCC	50.0	55.0	58.0	57.9	1018-1048 bp	1.0	84.2
ndhC-trnV-UAC_p2	TCTGGTCCTTCACTAGTCGG	GGTTCGAATCCGTATAGCCC	55.0	55.0	58.2	57.9	1046-1076 bp	1.0	83.0
ndhC-trnV-UAC_p3	GCCCTGAAATCCAAAATGCC	CGGTTCGAATCCGTATAGCC	50.0	55.0	58.0	58.3	1019-1049 bp	1.0	82.3
ndhC-trnV-UAC_p4	CACTAGTCGGGGCTAAAAGC	GGTTCGAATCCGTATAGCCC	55.0	55.0	58.3	57.9	1036-1066 bp	1.0	81.5
ndhC-trnV-UAC_p5	TCTGGTCCTTCACTAGTCGG	CGGTTCGAATCCGTATAGCC	55.0	55.0	58.2	58.3	1047-1077 bp	1.0	81.1