Hotspot

trnS-GCU-psbD

LSC · 7649-8620 · 972 bp

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Pi 0.00961

MarkerSeek score 64.10

Primer available yes

Label trnS-GCU-psbD

Feature Metadata

label_name: trnS-GCU-psbD
feature_type: igs
region: LSC
length_bp: 972
variable_sites: 14
pi: 0.00961
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 64.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnS-GCU-psbD_p1	ATTAGCAATCCGCCGCTTTA	GAATCGGTCCCTTCGTAACC	45.0	55.0	58.0	58.1	1096-1100 bp	1.0	80.2
trnS-GCU-psbD_p2	ATTAGCAATCCGCCGCTTTA	GCCGGACCATCCTACAAAAA	45.0	50.0	58.0	58.2	1117-1121 bp	1.0	79.4
trnS-GCU-psbD_p3	ATTAGCAATCCGCCGCTTTA	ACAAAAACGAATCGGTCCCT	45.0	45.0	58.0	57.7	1104-1108 bp	1.0	78.7
trnS-GCU-psbD_p4	GAGCGGAAAGAGAGGGATTC	GAATCGGTCCCTTCGTAACC	55.0	55.0	57.8	58.1	1147-1151 bp	1.0	78.6
trnS-GCU-psbD_p5	ATTAGCAATCCGCCGCTTTA	CCCTTCGTAACCAGTCATCC	45.0	55.0	58.0	57.7	1088-1092 bp	1.0	78.3