Hotspot

ycf1

IRb · 113204-114139 · 936 bp

Back to Tripterygium

Pi 0
MarkerSeek score 25.80
Primer available yes
Label ycf1

Feature Metadata

label_name
ycf1
feature_type
gene
region
IRb
length_bp
936
variable_sites
0
pi
0
unique_haplotype_count
1
primer_available
yes
alignment_reliability
1.00
markerseek_score
25.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ycf1_p1 GAGCTACTGAGGAACAACGG AGGGGGAGGGACTGGATATA 55.0 55.0 58.0 58.1 1843-1853 bp 1.0 79.4
ycf1_p2 AAGACAGAGGGAACGGGTTA AGGGGGAGGGACTGGATATA 50.0 55.0 58.0 58.1 1986-1996 bp 1.0 79.4
ycf1_p3 ATCTCCCCAGGTAGGATTCG AGGGGGAGGGACTGGATATA 55.0 55.0 58.0 58.1 1904-1914 bp 1.0 79.3
ycf1_p4 GAGCTACTGAGGAACAACGG AGGTGGTCGTATCTCTTCGT 55.0 50.0 58.0 57.9 1407 bp 0.5 59.0
ycf1_p5 ATCTCCCCAGGTAGGATTCG AGGTGGTCGTATCTCTTCGT 55.0 50.0 58.0 57.9 1468 bp 0.5 58.9