Hotspot

rps16

LSC · 4932-5225 · 294 bp

Back to Tripterygium

Pi 0.0034

MarkerSeek score 40.70

Primer available yes

Label rps16

Feature Metadata

label_name: rps16
feature_type: gene
region: LSC
length_bp: 294
variable_sites: 1
pi: 0.0034
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 40.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps16_p1	AAAAGCTGCTAGTCGCACTT	GATGCGCTTTTCATCCCATG	45.0	50.0	58.1	57.9	1619-1620 bp	1.0	78.3
rps16_p2	AAAGCCGAGTACTCTACCGT	GATGCGCTTTTCATCCCATG	50.0	50.0	58.2	57.9	1598-1599 bp	1.0	77.9
rps16_p3	AAGCCGAGTACTCTACCGTT	GATGCGCTTTTCATCCCATG	50.0	50.0	58.2	57.9	1597-1598 bp	1.0	77.9
rps16_p4	AAAAGCTGCTAGTCGCACTT	AAAAGCTTACGCCCACATCT	45.0	45.0	58.1	57.8	1555-1556 bp	1.0	77.7
rps16_p5	AAGCCGAGTACTCTACCGTT	AAAAGCTTACGCCCACATCT	50.0	45.0	58.2	57.8	1533-1534 bp	1.0	77.3