Hotspot

psbA

LSC · 478-1539 · 1062 bp

Back to Tripterygium

Pi 0.00282

MarkerSeek score 49.50

Primer available yes

Label psbA

Feature Metadata

label_name: psbA
feature_type: gene
region: LSC
length_bp: 1062
variable_sites: 3
pi: 0.00282
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 49.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbA_p1	CGAACTCGTATACACTAAAACCG	TGCTTGGGAGTCCCTGATAA	43.5	50.0	57.4	58.0	1135 bp	1.0	51.2
psbA_p2	AAACCGAACTCGTATACACT	TGCTTGGGAGTCCCTGATAA	40.0	50.0	54.2	58.0	1139 bp	1.0	49.3
psbA_p3	CCGAACTCGTATACACTAAAACC	TGCTTGGGAGTCCCTGATAA	43.5	50.0	57.1	58.0	1136 bp	1.0	48.4
psbA_p4	AAAACCGAACTCGTATACACT	TGCTTGGGAGTCCCTGATAA	38.1	50.0	55.0	58.0	1140 bp	1.0	48.1
psbA_p5	AAAAACCGAACTCGTATACACT	TGCTTGGGAGTCCCTGATAA	36.4	50.0	55.8	58.0	1141 bp	1.0	46.3