Hotspot

ccsA

SSC · 118155-119126 · 972 bp

Back to Tripterygium

Pi 0.00514

MarkerSeek score 55.10

Primer available yes

Label ccsA

Feature Metadata

label_name: ccsA
feature_type: gene
region: SSC
length_bp: 972
variable_sites: 5
pi: 0.00514
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 55.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ccsA_p1	AGTAGCGGCATGGCAATTTA	CGGCGTTTACACACAGTTTC	45.0	50.0	57.9	58.0	1160 bp	1.0	79.8
ccsA_p2	AGTAGCGGCATGGCAATTTA	CTGTCAAACGGCGTTTACAC	45.0	50.0	57.9	58.0	1168 bp	1.0	79.8
ccsA_p3	GCGGCATGGCAATTTATACC	CGGCGTTTACACACAGTTTC	50.0	50.0	57.9	58.0	1156 bp	1.0	79.7
ccsA_p4	GCGGCATGGCAATTTATACC	CTGTCAAACGGCGTTTACAC	50.0	50.0	57.9	58.0	1164 bp	1.0	79.7
ccsA_p5	AGTAGCGGCATGGCAATTTA	TCTGTCAAACGGCGTTTACA	45.0	45.0	57.9	58.1	1169 bp	1.0	79.4