Hotspot

atpF

LSC · 11656-12890 · 1235 bp

Back to Tripterygium

Pi 0.00406

MarkerSeek score 51.10

Primer available yes

Label atpF

Feature Metadata

label_name: atpF
feature_type: gene
region: LSC
length_bp: 1235
variable_sites: 5
pi: 0.00406
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 51.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
atpF_p1	GGCTCGAATGGTTACCATGA	AGTCGACAAATAGAGAATTAGTCT	50.0	33.3	57.7	55.2	1362-1366 bp	1.0	40.6
atpF_p2	GGCTCGAATGGTTACCATGA	AAGTCGACAAATAGAGAATTAGTCT	50.0	32.0	57.7	55.8	1363-1367 bp	1.0	40.6
atpF_p3	ACTAATTTCGTCGGCTCGAA	AGTCGACAAATAGAGAATTAGTCT	45.0	33.3	57.4	55.2	1374-1378 bp	1.0	40.6
atpF_p4	GGCTCGAATGGTTACCATGA	AGTCGACAAATAGAGAATTAGTC	50.0	34.8	57.7	53.7	1362-1366 bp	1.0	40.6
atpF_p5	GGCTCGAATGGTTACCATGA	GTCGACAAATAGAGAATTAGTCT	50.0	34.8	57.7	53.7	1361-1365 bp	1.0	40.6