Hotspot

ycf1-ndhF

SSC · 114140-114170 · 31 bp

Back to Tripterygium

Pi 0.185

MarkerSeek score 80.50

Primer available yes

Label ycf1-ndhF

Feature Metadata

label_name: ycf1-ndhF
feature_type: igs
region: SSC
length_bp: 31
variable_sites: 5
pi: 0.185
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.87
markerseek_score: 80.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf1-ndhF_p1	GGAACAAGAGGAATCCAACGA	TTGTATTTGTTTGCTGTATTCA	47.6	27.3	57.9	52.6	191-201 bp	1.0	43.7
ycf1-ndhF_p2	GGAACAAGAGGAATCCAACGA	CTCTTATTGTATTTGTTTGCTGT	47.6	30.4	57.9	53.6	197-207 bp	1.0	43.6
ycf1-ndhF_p3	GGAACAAGAGGAATCCAACGA	TCTCTTATTGTATTTGTTTGCTGT	47.6	29.2	57.9	55.0	198-208 bp	1.0	43.5
ycf1-ndhF_p4	AGGAACAAGAGGAATCCAACG	TCTCTTATTGTATTTGTTTGCTGT	47.6	29.2	57.9	55.0	199-209 bp	1.0	43.5
ycf1-ndhF_p5	AGGAACAAGAGGAATCCAACG	CTCTTATTGTATTTGTTTGCTGT	47.6	30.4	57.9	53.6	198-208 bp	1.0	43.5