Hotspot

rpl14-rpl16

LSC · 84495-84635 · 141 bp

Back to Tripterygium

Pi 0.0214

MarkerSeek score 65.50

Primer available yes

Label rpl14-rpl16

Feature Metadata

label_name: rpl14-rpl16
feature_type: igs
region: LSC
length_bp: 141
variable_sites: 3
pi: 0.0214
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 65.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl14-rpl16_p1	TCCGCCACATTCAAATGAGT	AGCGGCTTCAAAAATGCCTA	45.0	45.0	57.8	58.5	225 bp	1.0	76.2
rpl14-rpl16_p2	TCCGCCACATTCAAATGAGT	TAGCGGCTTCAAAAATGCCT	45.0	45.0	57.8	58.5	226 bp	1.0	76.2
rpl14-rpl16_p3	TCCGCCACATTCAAATGAGT	GCGGCTTCAAAAATGCCTAT	45.0	45.0	57.8	57.4	224 bp	1.0	75.2
rpl14-rpl16_p4	TACACATCAATTCTCGGGCC	AGCGGCTTCAAAAATGCCTA	50.0	45.0	57.7	58.5	254 bp	1.0	75.0
rpl14-rpl16_p5	TACACATCAATTCTCGGGCC	TAGCGGCTTCAAAAATGCCT	50.0	45.0	57.7	58.5	255 bp	1.0	75.0