Hotspot

petA-psbJ

LSC · 66161-67065 · 905 bp

Back to Tripterygium

Pi 0.00552

MarkerSeek score 61.90

Primer available yes

Label petA-psbJ

Feature Metadata

label_name: petA-psbJ
feature_type: igs
region: LSC
length_bp: 905
variable_sites: 5
pi: 0.00552
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 61.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
petA-psbJ_p1	ACGTGTCCAAGGTCTTTTGT	ATTCTGGATTGGGCTCATCC	45.0	50.0	57.6	57.3	1043-1050 bp	1.0	71.7
petA-psbJ_p2	CAGTACGTGTCCAAGGTCTT	ATTCTGGATTGGGCTCATCC	50.0	50.0	57.2	57.3	1047-1054 bp	1.0	68.5
petA-psbJ_p3	CCAGTACGTGTCCAAGGTCT	ATTCTGGATTGGGCTCATCC	55.0	50.0	59.0	57.3	1048-1055 bp	1.0	66.7
petA-psbJ_p4	CAGTACGTGTCCAAGGTCTTT	ATTCTGGATTGGGCTCATCC	47.6	50.0	57.9	57.3	1047-1054 bp	1.0	66.1
petA-psbJ_p5	CGCATCAGTTATTTTGGCACA	ATTCTGGATTGGGCTCATCC	42.9	50.0	57.8	57.3	1016-1023 bp	1.0	65.3