Hotspot

rbcL-accD

LSC · 60249-60846 · 598 bp

Back to Tripterygium

Pi 0.00502

MarkerSeek score 61.60

Primer available yes

Label rbcL-accD

Feature Metadata

label_name: rbcL-accD
feature_type: igs
region: LSC
length_bp: 598
variable_sites: 3
pi: 0.00502
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 61.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rbcL-accD_p1	GAGGCTAGCAAATGGAGTCC	CCGCATCGGTATTCTACCTT	55.0	50.0	58.0	57.2	747 bp	1.0	73.5
rbcL-accD_p2	GAGTCCTGAACTAGCTGCTG	CCGCATCGGTATTCTACCTT	55.0	50.0	57.7	57.2	733 bp	1.0	71.5
rbcL-accD_p3	GGCTAGCAAATGGAGTCCTG	CCGCATCGGTATTCTACCTT	55.0	50.0	58.3	57.2	745 bp	1.0	71.2
rbcL-accD_p4	AGGCTAGCAAATGGAGTCCT	CCGCATCGGTATTCTACCTT	50.0	50.0	58.4	57.2	746 bp	1.0	70.5
rbcL-accD_p5	CGAGGCTAGCAAATGGAGTC	CCGCATCGGTATTCTACCTT	55.0	50.0	58.4	57.2	748 bp	1.0	70.4