Hotspot

trnF-GAA-ndhJ

LSC · 51924-52736 · 813 bp

Back to Tripterygium

Pi 0.00496

MarkerSeek score 62.30

Primer available yes

Label trnF-GAA-ndhJ

Feature Metadata

label_name: trnF-GAA-ndhJ
feature_type: igs
region: LSC
length_bp: 813
variable_sites: 4
pi: 0.00496
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 62.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnF-GAA-ndhJ_p1	GGGATAGCTCAGCTGGTAGA	CCCGAAAGTTGGATAGGGTG	55.0	55.0	58.0	58.0	961-971 bp	1.0	80.5
trnF-GAA-ndhJ_p2	GGGATAGCTCAGCTGGTAGA	CCGAAAGTTGGATAGGGTGG	55.0	55.0	58.0	58.0	960-970 bp	1.0	80.5
trnF-GAA-ndhJ_p3	CCTCGTGTCACCAGTTCAAA	CCCGAAAGTTGGATAGGGTG	50.0	55.0	58.1	58.0	925-935 bp	1.0	80.1
trnF-GAA-ndhJ_p4	CCTCGTGTCACCAGTTCAAA	CCGAAAGTTGGATAGGGTGG	50.0	55.0	58.1	58.0	924-934 bp	1.0	80.1
trnF-GAA-ndhJ_p5	TATGCGTTGAGAATGGTCGG	CCGAAAGTTGGATAGGGTGG	50.0	55.0	58.1	58.0	978-988 bp	1.0	80.0