Hotspot

trnH-GUG-psbA

LSC · 67-477 · 411 bp

Back to Tripterygium

Pi 0.0122
MarkerSeek score 61.80
Primer available yes
Label trnH-GUG-psbA

Feature Metadata

label_name
trnH-GUG-psbA
feature_type
igs
region
LSC
length_bp
411
variable_sites
5
pi
0.0122
unique_haplotype_count
2
primer_available
yes
alignment_reliability
1.00
markerseek_score
61.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnH-GUG-psbA_p1 GATCCACTTGGCTACATCCG CTACTGTCGAAGTTCCAGCC 55.0 55.0 58.1 58.0 465-473 bp 1.0 79.8
trnH-GUG-psbA_p2 CAATCCACTGCCTTGATCCA CTACTGTCGAAGTTCCAGCC 50.0 55.0 57.9 58.0 479-487 bp 1.0 79.7
trnH-GUG-psbA_p3 AATCCACTGCCTTGATCCAC CTACTGTCGAAGTTCCAGCC 50.0 55.0 57.9 58.0 478-486 bp 1.0 79.7
trnH-GUG-psbA_p4 ACAATCCACTGCCTTGATCC CTACTGTCGAAGTTCCAGCC 50.0 55.0 57.9 58.0 480-488 bp 1.0 79.7
trnH-GUG-psbA_p5 GATCCACAATCCACTGCCTT CTACTGTCGAAGTTCCAGCC 50.0 55.0 57.9 58.0 485-493 bp 1.0 79.7