Hotspot

ycf1

SSC · 121168-126675 · 5508 bp

Back to Tripterospermum

Pi 0.00274

MarkerSeek score 42.80

Primer available yes

Label ycf1

Feature Metadata

label_name: ycf1
feature_type: gene
region: SSC
length_bp: 5508
variable_sites: 35
pi: 0.00274
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 42.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf1_p1	TCGACCTTGATCCAGTACTCA	GCAAGGATCTGACTGCTAGG	47.6	55.0	57.9	57.8	1171 bp	1.0	70.5
ycf1_p2	TCGACCTTGATCCAGTACTCA	CTGACTGCTAGGGCAATCAC	47.6	55.0	57.9	58.3	1163 bp	1.0	69.7
ycf1_p3	AGTCTAGGGTTGAGATGGGAC	GCAAGGATCTGACTGCTAGG	52.4	55.0	58.2	57.8	1365 bp	1.0	69.2
ycf1_p4	AGTCTAGGGTTGAGATGGGA	GCAAGGATCTGACTGCTAGG	50.0	55.0	56.7	57.8	1365 bp	1.0	69.2
ycf1_p5	GTCTAGGGTTGAGATGGGAC	GCAAGGATCTGACTGCTAGG	55.0	55.0	56.7	57.8	1364 bp	1.0	68.9