Hotspot

rbcL

LSC · 54744-56201 · 1458 bp

Back to Tripterospermum

Pi 0.00274

MarkerSeek score 47.50

Primer available yes

Label rbcL

Feature Metadata

label_name: rbcL
feature_type: gene
region: LSC
length_bp: 1458
variable_sites: 8
pi: 0.00274
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 47.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rbcL_p1	ACCCCACCGACTTTCTTTTC	AAAAAGCGTGCTTTCCCCTA	50.0	45.0	58.0	58.0	2346-2371 bp	1.0	80.6
rbcL_p2	ACCCCACCGACTTTCTTTTC	GCGTGCTTTCCCCTATGTTA	50.0	50.0	58.0	58.0	2341-2366 bp	1.0	80.4
rbcL_p3	CCCACCGACTTTCTTTTCGA	AAAAAGCGTGCTTTCCCCTA	50.0	45.0	58.1	58.0	2344-2369 bp	1.0	79.8
rbcL_p4	CCCACCGACTTTCTTTTCGA	GCGTGCTTTCCCCTATGTTA	50.0	50.0	58.1	58.0	2339-2364 bp	1.0	79.6
rbcL_p5	ACCCCACCGACTTTCTTTTC	AAAAGCGTGCTTTCCCCTAT	50.0	45.0	58.0	57.8	2345-2370 bp	1.0	79.0