Hotspot

rrn5-trnR-ACG

IRb · 105939-106199 · 261 bp

Back to Tripterospermum

Pi 0.00153

MarkerSeek score 38.70

Primer available yes

Label rrn5-trnR-ACG

Feature Metadata

label_name: rrn5-trnR-ACG
feature_type: igs
region: IRb
length_bp: 261
variable_sites: 1
pi: 0.00153
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 38.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rrn5-trnR-ACG_p1	GGTGACGATACTGTAGGGGA	CTTTTGGTCCGCTATTCCCA	55.0	50.0	57.9	57.9	421 bp	1.0	78.7
rrn5-trnR-ACG_p2	GGTGACGATACTGTAGGGGA	CCACGTGCTCTAATCCTCTG	55.0	55.0	57.9	57.8	345 bp	1.0	78.0
rrn5-trnR-ACG_p3	ATTCTGGTGTCCTAGGCGTA	CTTTTGGTCCGCTATTCCCA	50.0	50.0	57.8	57.9	489 bp	1.0	77.9
rrn5-trnR-ACG_p4	GTAGAGGAACCACACCAACC	CTTTTGGTCCGCTATTCCCA	55.0	50.0	57.8	57.9	472 bp	1.0	77.7
rrn5-trnR-ACG_p5	GGTGACGATACTGTAGGGGA	AGCTTTTGGTCCGCTATTCC	55.0	50.0	57.9	58.2	423 bp	1.0	77.7