Hotspot

rrn4.5-rrn5

IRb · 105574-105817 · 244 bp

Back to Tripterospermum

Pi 0.0213

MarkerSeek score 52.60

Primer available yes

Label rrn4.5-rrn5

Feature Metadata

label_name: rrn4.5-rrn5
feature_type: igs
region: IRb
length_bp: 244
variable_sites: 13
pi: 0.0213
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 52.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rrn4.5-rrn5_p1	CAGCTGAGGCATCCTAACAG	AGTATCGTCACCGCAGTAGA	55.0	50.0	58.0	58.0	361 bp	1.0	82.2
rrn4.5-rrn5_p2	CAGCTGAGGCATCCTAACAG	TATCGTCACCGCAGTAGAGT	55.0	50.0	58.0	58.0	359 bp	1.0	82.2
rrn4.5-rrn5_p3	GCTGAGGCATCCTAACAGAC	TATCGTCACCGCAGTAGAGT	55.0	50.0	58.1	58.0	357 bp	1.0	82.2
rrn4.5-rrn5_p4	GTCAAGTGGAAGTGCAGTGA	TATCGTCACCGCAGTAGAGT	50.0	50.0	58.0	58.0	385 bp	1.0	82.1
rrn4.5-rrn5_p5	GTCAAGTGGAAGTGCAGTGA	AGTATCGTCACCGCAGTAGA	50.0	50.0	58.0	58.0	387 bp	1.0	82.0