Hotspot

psaJ-rpl33

LSC · 66112-66610 · 499 bp

Back to Tripterospermum

Pi 0.00292

MarkerSeek score 43.10

Primer available yes

Label psaJ-rpl33

Feature Metadata

label_name: psaJ-rpl33
feature_type: igs
region: LSC
length_bp: 499
variable_sites: 3
pi: 0.00292
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 0.82
markerseek_score: 43.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psaJ-rpl33_p1	GGCACCAGTACTAAGTACGC	GGCACAACTGGTACATTCCA	55.0	50.0	58.1	58.1	574-662 bp	1.0	83.0
psaJ-rpl33_p2	GGCACCAGTACTAAGTACGC	TAACGCTATTTCGGGCACAA	55.0	45.0	58.1	57.9	587-675 bp	1.0	83.0
psaJ-rpl33_p3	GGCACCAGTACTAAGTACGC	TTAACGCTATTTCGGGCACA	55.0	45.0	58.1	57.9	588-676 bp	1.0	83.0
psaJ-rpl33_p4	TGCGCTAACATTCCCCTTTT	GGCACAACTGGTACATTCCA	45.0	50.0	58.1	58.1	499-587 bp	1.0	83.0
psaJ-rpl33_p5	TGCGCTAACATTCCCCTTTT	TAACGCTATTTCGGGCACAA	45.0	45.0	58.1	57.9	512-600 bp	1.0	83.0