Hotspot

petA-psbJ

LSC · 62408-63000 · 593 bp

Back to Tripterospermum

Pi 0.00485

MarkerSeek score 57.30

Primer available yes

Label petA-psbJ

Feature Metadata

label_name: petA-psbJ
feature_type: igs
region: LSC
length_bp: 593
variable_sites: 6
pi: 0.00485
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 57.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
petA-psbJ_p1	CCCATTACGTGTCCAAGGAC	CATATTCCGGATTGGGGTCA	55.0	50.0	58.3	57.1	734-740 bp	1.0	72.6
petA-psbJ_p2	GACCCATTACGTGTCCAAGG	CATATTCCGGATTGGGGTCA	55.0	50.0	58.3	57.1	736-742 bp	1.0	72.6
petA-psbJ_p3	ACGTGTCCAAGGACTTTTGT	CATATTCCGGATTGGGGTCA	45.0	50.0	57.6	57.1	728-734 bp	1.0	71.4
petA-psbJ_p4	CCCATTACGTGTCCAAGGAC	TGGTTCATATTCCGGATTGGG	55.0	47.6	58.3	57.8	739-745 bp	1.0	70.4
petA-psbJ_p5	GACCCATTACGTGTCCAAGG	TGGTTCATATTCCGGATTGGG	55.0	47.6	58.3	57.8	741-747 bp	1.0	70.4