Hotspot

accD-psaI

LSC · 58369-58904 · 536 bp

Back to Tripterospermum

Pi 0.00455

MarkerSeek score 38.50

Primer available yes

Label accD-psaI

Feature Metadata

label_name: accD-psaI
feature_type: igs
region: LSC
length_bp: 536
variable_sites: 6
pi: 0.00455
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 38.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
accD-psaI_p1	TTCCACGCTTTCTTTCCCTT	ACAAAAATAGAGGGAAAACTGA	45.0	31.8	57.6	53.5	616-770 bp	1.0	41.6
accD-psaI_p2	GTTCCACGCTTTCTTTCCCT	ACAAAAATAGAGGGAAAACTGA	50.0	31.8	58.4	53.5	617-771 bp	1.0	41.6
accD-psaI_p3	TTTGACCTAATCGTACCGCG	ACAAAAATAGAGGGAAAACTGA	50.0	31.8	58.1	53.5	673-827 bp	1.0	41.5
accD-psaI_p4	TTTGACCTAATCGTACCGCG	ACAAAAATAGAGGGAAAACTGAA	50.0	30.4	58.1	54.4	673-827 bp	1.0	41.5
accD-psaI_p5	TGACCTAATCGTACCGCGTA	ACAAAAATAGAGGGAAAACTGA	50.0	31.8	58.3	53.5	671-825 bp	1.0	41.5