Hotspot

trnF-GAA-ndhJ

LSC · 47852-48403 · 552 bp

Back to Tripterospermum

Pi 0.00533

MarkerSeek score 51.00

Primer available yes

Label trnF-GAA-ndhJ

Feature Metadata

label_name: trnF-GAA-ndhJ
feature_type: igs
region: LSC
length_bp: 552
variable_sites: 7
pi: 0.00533
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 0.95
markerseek_score: 51.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnF-GAA-ndhJ_p1	GGGATAGCTCAGCTGGTAGA	CCGGAAAGTTGGATAGGGTG	55.0	55.0	58.0	58.0	690-700 bp	1.0	83.6
trnF-GAA-ndhJ_p2	GGGATAGCTCAGCTGGTAGA	CGGAAAGTTGGATAGGGTGG	55.0	55.0	58.0	58.0	689-699 bp	1.0	83.6
trnF-GAA-ndhJ_p3	CCTCGTGTCACCAGTTCAAA	CGGAAAGTTGGATAGGGTGG	50.0	55.0	58.1	58.0	653-663 bp	1.0	83.4
trnF-GAA-ndhJ_p4	GGATGCTACATTGGGACTGG	CCGGAAAGTTGGATAGGGTG	55.0	55.0	58.0	58.0	712-722 bp	1.0	83.3
trnF-GAA-ndhJ_p5	GGATGCTACATTGGGACTGG	CGGAAAGTTGGATAGGGTGG	55.0	55.0	58.0	58.0	711-721 bp	1.0	83.3