Hotspot

trnC-GCA-petN

LSC · 27326-28061 · 736 bp

Back to Tripterospermum

Pi 0.00231

MarkerSeek score 55.50

Primer available yes

Label trnC-GCA-petN

Feature Metadata

label_name: trnC-GCA-petN
feature_type: igs
region: LSC
length_bp: 736
variable_sites: 4
pi: 0.00231
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 55.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnC-GCA-petN_p1	TTTCCCCAGTTCAAATCCGG	TTAAAGCAGCCCAAGCAAGA	50.0	45.0	58.1	58.0	581-799 bp	1.0	83.7
trnC-GCA-petN_p2	CCCAGTTCAAATCCGGGTAC	TTAAAGCAGCCCAAGCAAGA	55.0	45.0	58.3	58.0	577-795 bp	1.0	82.3
trnC-GCA-petN_p3	TTTCCCCAGTTCAAATCCGG	CAGCCCAAGCAAGACTTACT	50.0	50.0	58.1	57.8	575-793 bp	1.0	82.2
trnC-GCA-petN_p4	GGGGGACTGCAAATCCTTTT	TTAAAGCAGCCCAAGCAAGA	50.0	45.0	58.4	58.0	599-817 bp	1.0	81.5
trnC-GCA-petN_p5	CCCAGTTCAAATCCGGGTAC	CAGCCCAAGCAAGACTTACT	55.0	50.0	58.3	57.8	571-789 bp	1.0	80.9