Hotspot

rpoB-trnC-GCA

LSC · 25963-27254 · 1292 bp

Back to Tripterospermum

Pi 0.00255

MarkerSeek score 58.70

Primer available yes

Label rpoB-trnC-GCA

Feature Metadata

label_name: rpoB-trnC-GCA
feature_type: igs
region: LSC
length_bp: 1292
variable_sites: 8
pi: 0.00255
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 0.97
markerseek_score: 58.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpoB-trnC-GCA_p1	GTTCTTCCGTCAAACCCTCA	TTTTATCAGCCGACGAAGGG	50.0	50.0	57.8	58.0	1402-1506 bp	1.0	82.3
rpoB-trnC-GCA_p2	GTTCTTCCGTCAAACCCTCA	CCGGATTTGAACTGGGGAAA	50.0	50.0	57.8	58.1	1354-1457 bp	1.0	81.8
rpoB-trnC-GCA_p3	GTTCTTCCGTCAAACCCTCA	GACGAAGGGATTTCAAGGCT	50.0	50.0	57.8	57.9	1391-1495 bp	1.0	81.5
rpoB-trnC-GCA_p4	CTTCCGTCAAACCCTCATCA	TTTTATCAGCCGACGAAGGG	50.0	50.0	57.5	58.0	1399-1503 bp	1.0	80.5
rpoB-trnC-GCA_p5	GTTCTTCCGTCAAACCCTCA	GGTACCCGGATTTGAACTGG	50.0	55.0	57.8	58.3	1359-1462 bp	1.0	80.4