Hotspot

ndhH

SSC · 117120-118301 · 1182 bp

Back to Tripsacum

Pi 0.00169

MarkerSeek score 59.70

Primer available yes

Label ndhH

Feature Metadata

label_name: ndhH
feature_type: gene
region: SSC
length_bp: 1182
variable_sites: 2
pi: 0.00169
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 59.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhH_p1	GTAGGAGGGCAAAAATGGGT	TGCATTTTGATGAGCCTCGT	50.0	45.0	57.8	58.2	1368 bp	1.0	76.9
ndhH_p2	GGAGGGCAAAAATGGGTAGA	TGCATTTTGATGAGCCTCGT	50.0	45.0	57.5	58.2	1365 bp	1.0	74.5
ndhH_p3	AGGAGGGCAAAAATGGGTAG	TGCATTTTGATGAGCCTCGT	50.0	45.0	57.5	58.2	1366 bp	1.0	74.5
ndhH_p4	AGTAGGAGGGCAAAAATGGG	TGCATTTTGATGAGCCTCGT	50.0	45.0	57.5	58.2	1369 bp	1.0	74.5
ndhH_p5	CCCAGTAGGAGGGCAAAAAT	TGCATTTTGATGAGCCTCGT	50.0	45.0	57.5	58.2	1372 bp	1.0	74.5