Hotspot

atpB-rbcL

LSC · 56671-57491 · 821 bp

Back to Tripsacum

Pi 0.00244
MarkerSeek score 60.40
Primer available yes
Label atpB-rbcL

Feature Metadata

label_name
atpB-rbcL
feature_type
igs
region
LSC
length_bp
821
variable_sites
2
pi
0.00244
unique_haplotype_count
2
primer_available
yes
alignment_reliability
1.00
markerseek_score
60.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
atpB-rbcL_p1 AATTGTGGAAATCCCGGGAC ACTCCGGGGTGTAGTAAGTC 50.0 55.0 58.2 58.2 950-951 bp 1.0 77.9
atpB-rbcL_p2 AATTGTGGAAATCCCGGGAC CTCCGGGGTGTAGTAAGTCA 50.0 55.0 58.2 58.2 949-950 bp 1.0 77.9
atpB-rbcL_p3 CAATTGTGGAAATCCCGGGA ACTCCGGGGTGTAGTAAGTC 50.0 55.0 58.2 58.2 951-952 bp 1.0 77.9
atpB-rbcL_p4 TCAATTGTGGAAATCCCGGG ACTCCGGGGTGTAGTAAGTC 50.0 55.0 58.2 58.2 952-953 bp 1.0 77.9
atpB-rbcL_p5 CAATTGTGGAAATCCCGGGA CTCCGGGGTGTAGTAAGTCA 50.0 55.0 58.2 58.2 950-951 bp 1.0 77.9