Hotspot

ndhC-trnV

LSC · 52766-53714 · 949 bp

Back to Tripsacum

Pi 0.00211

MarkerSeek score 60.00

Primer available yes

Label ndhC-trnV

Feature Metadata

label_name: ndhC-trnV
feature_type: igs
region: LSC
length_bp: 949
variable_sites: 2
pi: 0.00211
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 60.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhC-trnV_p1	CTAACCGGGGCTAAAAGTCC	GGTTCGAATCCGTATAGCCC	55.0	55.0	58.0	57.9	1075 bp	1.0	79.3
ndhC-trnV_p2	CTAACCGGGGCTAAAAGTCC	AGCCCAATGCAGAAAAATGC	55.0	45.0	58.0	57.9	1168 bp	1.0	79.0
ndhC-trnV_p3	CTAACCGGGGCTAAAAGTCC	CGGTTCGAATCCGTATAGCC	55.0	55.0	58.0	58.3	1076 bp	1.0	77.4
ndhC-trnV_p4	TAACCGGGGCTAAAAGTCCT	GGTTCGAATCCGTATAGCCC	50.0	55.0	58.3	57.9	1074 bp	1.0	76.8
ndhC-trnV_p5	TAACCGGGGCTAAAAGTCCT	AGCCCAATGCAGAAAAATGC	50.0	45.0	58.3	57.9	1167 bp	1.0	76.6