Hotspot

rps2-atpI

LSC · 33105-33354 · 250 bp

Back to Tripsacum

Pi 0.008

MarkerSeek score 57.10

Primer available yes

Label rps2-atpI

Feature Metadata

label_name: rps2-atpI
feature_type: igs
region: LSC
length_bp: 250
variable_sites: 2
pi: 0.008
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 57.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps2-atpI_p1	AATTTCTGAGGGCCGTTCTC	GCCAATAGAAGTGTTGCCCT	50.0	50.0	57.9	58.2	375 bp	1.0	78.2
rps2-atpI_p2	CAATTTCTGAGGGCCGTTCT	GCCAATAGAAGTGTTGCCCT	50.0	50.0	58.2	58.2	376 bp	1.0	77.8
rps2-atpI_p3	GATTCCTGCCAACGATGACA	GCCAATAGAAGTGTTGCCCT	50.0	50.0	58.3	58.2	438 bp	1.0	77.0
rps2-atpI_p4	CTGAGGGCCGTTCTCTCTAT	GCCAATAGAAGTGTTGCCCT	55.0	50.0	58.3	58.2	370 bp	1.0	76.7
rps2-atpI_p5	CCTGCCAACGATGACACTAT	GCCAATAGAAGTGTTGCCCT	50.0	50.0	57.7	58.2	434 bp	1.0	76.6