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trnC-rpoB

LSC · 20793-22033 · 1241 bp

Back to Tripsacum

Pi 0.00081

MarkerSeek score 59.80

Primer available yes

Label trnC-rpoB

Feature Metadata

label_name: trnC-rpoB
feature_type: igs
region: LSC
length_bp: 1241
variable_sites: 1
pi: 0.00081
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 59.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnC-rpoB_p1	AAAGGATTTGCAGTCCCCTG	CTTCTCAAGTTCTTCCGCCA	50.0	50.0	58.1	57.8	1388-1395 bp	1.0	78.5
trnC-rpoB_p2	AAAGGATTTGCAGTCCCCTG	TCTCAAGTTCTTCCGCCAAG	50.0	50.0	58.1	57.8	1386-1393 bp	1.0	78.5
trnC-rpoB_p3	AAAGGATTTGCAGTCCCCTG	ACTTCTCAAGTTCTTCCGCC	50.0	50.0	58.1	57.8	1389-1396 bp	1.0	78.5
trnC-rpoB_p4	AAAGGATTTGCAGTCCCCTG	TTCCGCCAAGCCTTGATTAA	50.0	45.0	58.1	57.8	1376-1383 bp	1.0	78.2
trnC-rpoB_p5	AAAGGATTTGCAGTCCCCTG	CTTCCGCCAAGCCTTGATTA	50.0	50.0	58.1	58.2	1377-1384 bp	1.0	77.9