Hotspot

trnD-psbM

LSC · 17727-18784 · 1058 bp

Back to Tripsacum

Pi 0.000954

MarkerSeek score 60.20

Primer available yes

Label trnD-psbM

Feature Metadata

label_name: trnD-psbM
feature_type: igs
region: LSC
length_bp: 1058
variable_sites: 1
pi: 0.000954
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 60.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnD-psbM_p1	AATAGGCATGCCATACACCC	GGCAGTAGGAACTAGAATGAACA	50.0	43.5	57.7	57.9	1213-1216 bp	1.0	53.7
trnD-psbM_p2	TTCAATTGGTCAGAGCACCG	GGCAGTAGGAACTAGAATGAACA	50.0	43.5	58.5	57.9	1179-1182 bp	1.0	52.2
trnD-psbM_p3	AATAGGCATGCCATACACCC	AGGCAGTAGGAACTAGAATGAAC	50.0	43.5	57.7	57.7	1214-1217 bp	1.0	51.6
trnD-psbM_p4	AATAGGCATGCCATACACCC	AGGCAGTAGGAACTAGAATGA	50.0	42.9	57.7	55.5	1214-1217 bp	1.0	50.4
trnD-psbM_p5	AATAGGCATGCCATACACCC	TGCGAGAATATTGACTTCCA	50.0	40.0	57.7	54.5	1174-1177 bp	1.0	50.2