Hotspot

trnS-psbD

LSC · 8485-9489 · 1005 bp

Back to Tripsacum

Pi 0.00199

MarkerSeek score 60.10

Primer available yes

Label trnS-psbD

Feature Metadata

label_name: trnS-psbD
feature_type: igs
region: LSC
length_bp: 1005
variable_sites: 2
pi: 0.00199
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 60.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnS-psbD_p1	ATTAGCAATCCGCCGCTTTA	ATAAGCCGGACCATCCTACA	45.0	50.0	58.0	57.9	1152-1154 bp	1.0	79.2
trnS-psbD_p2	ATTAGCAATCCGCCGCTTTA	TAAGCCGGACCATCCTACAA	45.0	50.0	58.0	58.1	1151-1153 bp	1.0	78.9
trnS-psbD_p3	ATTAGCAATCCGCCGCTTTA	GCCGGACCATCCTACAAAAA	45.0	50.0	58.0	58.2	1148-1150 bp	1.0	78.6
trnS-psbD_p4	ATTAGCAATCCGCCGCTTTA	ACAAAAACGAAACGGTCCCT	45.0	45.0	58.0	58.2	1135-1137 bp	1.0	78.0
trnS-psbD_p5	GCTTTAGTCCACTCAGCCAT	ATAAGCCGGACCATCCTACA	50.0	50.0	57.6	57.9	1138-1140 bp	1.0	76.2